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The present study was aimed to find out phytochemical testing and acute toxicity effect of the methanolic extract of Actiniopteris Dichotoma Bedd in rats. For phytochemical testing, depending upon the type of natural drug under examination, the test solution may be a aqueous extract or specific menstrum like petroleum ether, chloroform, methanol and aquous etc. All the different extracts were then subjected to preliminary phytochemical analysis to assess the presence of various phytoconstituents. The acute oral toxicity of the crude ethanolic extracts of Actiniopteris dichotoma was evaluated in mice using the procedures described by Organization for Economic Co-operation and Development 423 guidelines. The acute toxicity study showed that animals fed by oral gavages tolerated the limit dose of 5000 mg/kg body weight of methanolic extract of Actiniopteris dichotoma bedd. There were no visible signs of acute toxicity during the 14 days of observation.