Research Article
Open Access
Epigallocatechin gallate potentially ameliorates sodium fluoride-induced genotoxicity in rats.
S.Thangapandiyan and S.Milton prabu
Department of Zoology, Annamalai University, Annamalai nagar-608002.
S.Thangapandiyan and S.Milton prabu. Epigallocatechin gallate potentially ameliorates sodium fluoride-induced DNA damage, and genotoxicity in the bone marrow cells of rats. Asian Journal of Pharmacology and Toxicology 01 (02); 2013; 08-16.
Abstract
Fluoride compounds are naturally present in soil, water and food. The aim of this study was to investigate the possible ameliorative effect of Epigallocatechin gallate (EGCG) on sodium fluoride induced oxidative stress mediated genotoxicity in the bone marrow cells of rats. Rats were orally (gastric intubation) pretreated with EGCG (40mg/kg BW) followed by Sodium fluoride (NaF) (25mg/kg BW) daily for a period of 28days. NaF intoxicated rats showed a significant increase in the frequency of micronucleus (MN) in polychromatic erythrocytes (PCEs), structural chromosome aberrations (CA) with decreased mitotic index (MI) in bone marrow cells. We also found a significant increased DNA damage in bone marrow cells. Pre- treatment with EGCG (40mg/kg BW) significantly reduced the NaF induced genotoxicity as evidenced with the decreased micronucleus, chromosomal aberration and DNA damage, with increased mitotic index in rat bone marrow cells. In conclusion, the obtained data suggest that EGCG potentially protects bone marrow cells from NaF induced DNA damage and genotoxicity.
Keywords
EGCG, NaF, genotoxicity, rat, bone marrow, ros
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