The core finding of the present preclinical study is to develop and standardize the animal model of diabetic foot ulcers by using varying doses of Alloxan by using excision and incision wound ulcer technique.

Optimization with subcutaneous administration of the chemical inducer (Alloxan) through blood glucose and body weight monitoring of rat. Administration of prescribed oral and topical doses of Aloe Vera is remarkably protective effects in diabetic and its complication using male wistar rats against alloxan induced Diabetic Foot Ulcers (DFU). Diabetes mellitus is an endocrine disorder. Diabetes mellitus is now defined as a metabolic disorder in which the body’s capacity to utilize glucose, fat and protein is disturbed due to insulin deficiency, insulin resistance or both. Diabetes is a condition which is known to be associated with a variety of connective tissue abnormalities. The collagen content of the skin is decreased as a result of reduced biosynthesis and or accelerated degradation of newly synthesized collagen and epithelisation. These qualitative and quantitative abnormalities contribute to the impaired wound healing observed in diabetes. Aqueous extract of Aloe Vera (AGE, Oral) with ethanolic extract of Aloe Vera (AG, Topical) act as DFU protective agent. Relevant doses of Aloe Vera treatment significantly attenuated damage of rat against Alloxan induced connective tissue abnormality in diabetic wound ulcer. The histopathological examination and wound ulcer contraction results show that Aloe Vera treatment is effective in DFU models; it could be used as an effective therapeutic agent in the management of Diabetic foot ulcers and related conditions.