Introduction: Present study was designed to evaluate the protective effects of DL-α-Lipoic acid in aniline induced spleen toxicity in rats.

Material and Methods: Splenic toxicity was induced in rats by administration of aniline (30mg/kg/p.o) for a period of 7 days. Treatment groups received DL-α-Lipoic acid (40 mg/kg/day, p.o) for 7 days after aniline administration for 7 days i.e the study protocol was for 14 days. At the end of treatment period various serum and tissue parameters were evaluated. Rats administered with Aniline showed a significant alteration in general parameters such as organ weight, body weight, water intake, feed consumption and faecal matter content. It also alters hematological parameters (RBC, WBC and Hemoglobin content), biochemical parameters (total iron content, lipid peroxidation, reduced glutathione and nitric oxide content) and histopathology of spleen. Results: Treatment with DL-α-Lipoic acid for 7 days post aniline administeration showed significant recovery in aniline induced splenic toxicity. Conclusion: The present result showed that involvement of oxidative and nitrasative stress in aniline induced splenic toxicity and DL-α-Lipoic acid protects the rats from the toxicity which might be due to its strong antioxidant property.aniline; spleen toxicity; DL-α-Lipoic acid, antioxidants