Myocardial infarction (MI) is the interruption of blood supply to part of the heart, causing heart cells to die, commonly due to occlusion (blockage) of a coronary artery. Herbal drugs are known to exhibit creditable medicinal properties for the treatment of heart ailments and need to be explored to identify their potential application in prevention and therapy of human ailments. Considering this aspect, the study aimed to elucidate the cardio protective activity of Syzygium Aromaticum. Myocardial infarction was induced by a subcutaneous administration of isoproterenol. The positive inotropic and chronotropic response of isoproterenol caused a severe oxidative stress in the myocardium through increased lipid per oxidation. Extract of Syzygium Aromaticum was administered at a dose of 250, 500 and 750 mg/kg for 30 days. At the end of treatment period Isoproterenol 5.25 and 8.5 mg/kg s.c. was administered on two consecutive days (31th & 32nd day). haemodynamic parameters were recorded and the hearts were subsequently removed and processed for histopathological and biochemical studies. Histological examination of rat’s heart section confirmed myocardial injury with isoproterenol. Heart tissue enzyme analysis in albino (Wistar) male rats, such as LPO, GSH, GPX, GST, SOD, CAT, CK-MB, MDA and biochemical analysis in serum viz., ALT, AST, LDH, CPK were performed. Methanolic extract of Syzygium Aromaticum (CPE) at the dose of (250, 500 & 750 mg/kg) produced significant salvages the heart from isoproterenol induced myocardial ischemic injury. Syzygium Aromaticum contain eugenol, eugenol acetate, caryophyllene, sesquetrepene ester are having antioxidant, antilipid peroxidative, free radical scavenging properties and anti-ischemic activity justify its potential therapeutic value in the treatment of ischemic heart diseases in albino rats.