Cytogenetic bio-monitoring study in diabetes patients by Buccal cytome assay in exfoliated buccal epithelial cells
Diabetes mellitus is one of the most common metabolic disorders. It affects millions of people worldwide. Diabetes causes include cardiovascular disease, stroke, chronic kidney failure, foot ulcers, and damage to the eyes. The study was undertaken to compare the frequencies of nuclear abnormalities in buccal cells between diabetic and non-diabetic individuals. 20 diabetic and 20 non-diabetic individuals of both sexes participated in this parallel, randomized, intervention trial. Cytogenetic damage in buccal cells was assessed by using the buccal cytome (Bcyt) assay. It was observed that micronucleus (MN) frequency was significantly higher in participants with diabetes (8.62 + 0.52) compared with non-diabetic individuals (0.36+0.14). Significant increase was observed in other nuclear abnormalities e.g., binucleated cells (5.60+0.72) and cells with nuclear bud (6.40 + 0.59) in diabetic individuals in comparison to non-diabetic participants binucleated cells (0.46 + 0.18), nuclear buds (0.29 + 0.12). When we compared all the three parameters of Bcyt assay among diabetic and non-diabetic individuals, statistically significant (P<0.001) were observed. ><0.001) were observed.