Anvar Shakeri

ABSTRACT

Background: Awareness of antioxidantpotential of natural fruit, vegetables, medicinal plants etc. is rapidly developing world wide. Free oxidative radicals produced in our body by the process of natural metabolism cause the damage of healthy cells in our body. Antioxidants scavenge the free oxidative radicals and protect our healthy cells. Present study is an attempt to evaluate the antioxidant potential of Grape seed by estimating the total phenolic contents.Gallic acid, the specific bioactive compound present in grape seed, identified by HPLC technique, showed to have a genoprotective potential for prevention of oxidative DNA damage. Materials & methods: Fresh grape seeds in form of powder were used for methanol, ethanol and acetone solvent extraction. The specific bioactive compound present in grape seed extract (Gallic acid) was analytically separated and identified by HPLC technique. Antioxidant potential of Grape seed was checked on Yeast Model by Viable Cell Count method, which showed that grape seed extract prevents oxidative cell damage. Total Phenolic compounds present in grape seed was determined by Folin-Ciocalteu assay. The potential of scavenging oxidative radicals was proved by DPPH assay. FRAP assay determined the potential of grape seed extract to reduce ferric compounds to ferrous, which is considered to be an important property of being an anti-oxidant. Protection of oxidative DNA damage by grape seed extract was also shown by Agarose Gel Electrophoresis technique using plasmid DNA.