Thangappan Ramesh Kumar

ABSTRACT

The pesticides have different adverse impacts on different life stages of fish species with attempts to use dietary antioxidants to counteract their effects. Triclosan (TCS) is commonly used as antimicrobial agent that is incorporated into dish soap, detergent, toothpaste, mouthwash, shampoo, etc., in addition to innumerable other personal care and household products. Hence the current study was carried out to investigated the potential protective effects of garlic extract and L-Ascorbic acid (Vitamin C) and their combination versus triclosan-induced changes in zebra fish Brachydaniorerio (Hamilton). The median lethal concentration of triclosan for 96h is (0.32mg/L). The species were exposed to sub lethal concentration of triclosan (0.32mg/L) for 7 and 28 days. Triclosan significantly (P<0.05) increasesthe lipid peroxidation, supperoxide dismutase, enzyme catalase, and increases the serum glucose level. Addition of garlic extract and/or Vitamin C to the diet of triclosan-treated fish showed the increase level of the serum glucose, serum total protein, total lipid, creatinine, sodium, potassium, calcium, Blood urea nitrogen (BUN), uric acid, albumin and cholesterol , LPO, SOD, CAT, levels in comparison to control fish.